11/3/2022 0 Comments Parts of a microscope and function![]() These crews center the condenser, and therefore the beam of light. (L) Condenser control knobĪllows the condenser to be raised or lowered. As light travels from the illuminator, it passes through the condenser, where it is focused and directed at the specimen. The condenser is located under the stage. These knobs move the slide or specimen either horizontally (x-axis) or vertically (y-axis) when it is being viewed. Located just below the stage are the stage control knobs. Spring-loaded brackets, or clips, hold the slide or specimen in place on the stage. ![]() Figure 798.1: Compound microscope (I) Stage brackets The platform on which the slide or object is placed for viewing. Total magnification can be calculated by multiplying the objective power by the ocular lens power.įor example 100× objective lens with 10× oculars = 1000× total magnification. 10× objective is considered “low power,” 40× is “high power” and 100× objective is referred to as “oil immersion.” Once magnified by the objective lens, the image is viewed through the oculars, which magnifies it further. 4× objective is used mainly for scanning. Objective lenses are available in many powers, but 4×, 10×, 40×, and 100× are standard. The objective lens is the lens closest to the object being viewed, and its function is to magnify it. Most nosepieces can hold up to five objectives. The objectives are mounted on a rotating turret so they can be moved into place as needed. The nosepiece holds the objective lenses. ![]() Using one eye can cause eye strain over a period of time. When viewing a sample, always use both eyes. When looking through the microscope, one should see only a single field of view. This allows the oculars to move closer or further away from one another to match the width of an individual’s eyes. The purpose of the diopter adjustment is to correct the differences in a vision an individual may have between their left and right eyes. Oculars come in different magnifications, but 10× magnification is common. The total magnification can be calculated by multiplying the objective power by the ocular power. The lens of the microscope you look through. ![]()
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